产品中心Product center

scarpa

Amresco N806Ready PCR Mix

产品编号:
市场价:¥0.00
会员价:¥0.00
品牌:中文
生产厂家:Amresco
需求数量:
提交询价

Ready PCR Mix is a PCR master mix that enables reaction set-up through product visualization with a single, easy-to-use solution. With Ready PCR Mix, your laboratory can achieve high quality DNA amplification while slashing time and consumption of supplies and hazardous reagents. Ready PCR Mix can:
  • Reduce PCR reaction set up and post PCR steps
  • Streamline colony screening by eliminating the need for DNA purification
  • Eliminate Ethidium Bromide staining and associated waste disposal expenses

The Ready PCR Mix solution includes:

  • Extender™ Taq DNA Polymerase
    • High fidelity polymerase blend
    • Efficient amplification of long and challenging templates (To learn more, click on the features tab of the linked page)
  • Reaction Buffer
    • MgCl2
    • dNTPs
  • Density Reagent/Tracking Dye
    • Load reaction aliquots directly onto gel
    • Non-interfering, fast migrating dye
  • Fluorescent DNA Dye
    • Based on AMRESCO's non-mutagenic and non-toxic EZ-Vision™ formulation
    • Imte visualization of amplified product with no post-run staining or destaining
    • Eliminates use of Ethidium Bromide and associated hazardous waste disposal costs

Efficient Amplification of Purified DNA with Ready PCR Mix



Figure 1. Single copy targets were amplified from 50ng of pUC19 plasmid (A) or S. aureus genomic DNA (B) for 35 cycles. 10μl of each PCR reaction was directly loaded onto a 1% agarose gel and visualized with a Syngene HR gel doc system.
 
 
 
 

Direct Colony Screening with Ready PCR Mix

 
Figure 2. 10 distinct colonies were suspended into Ready PCR Mix in separate PCR tubes. 5 μl of the PCR reaction mix was plated on gridded LB plates with Kanamycin for 37ºC overnight growth. After 30 PCR cycles products were screened on a 1% agarose gel. Colonies 4, 8, 9 and 10 contained the desired sequences. Courtesy of Dr. Irene Lee, Case Western Reserve University, Cleveland, OH
 
 
 
 

DNA Amplification by Ready PCR Mix Outperforms a Competitor Master Mix

 
Figure 3. Amplification products from AMRESCO Ready PCR Mix (Lanes 5-8) and a competitor master mix (Lanes 1-4) were resolved on a TAE/1.5%  agarose gel containing Ethidium Bromide. Ethidium Bromide was included in the gel to visualize the products from the competitor master mix which lacks a DNA dye. Lanes 1, 2, 3:  Aliquots (5 µl)  from triplicate reactions with competitor master mix. Lanes  5,6,7: Aliquots (5 µl)  from triplicate reactions with AMRESCO Ready PCR mix. Lanes 4 & 8: Non-template controls (5 µl) from competitor master mix and AMRESCO Ready PCR mix respectively. Data courtesy of Norgen Biotek Corporation.

请填写以下表格,我们会尽快与您联系!或者直接致电400 628 5117,谢谢!

* 您的姓名: *
部门及职位:
* 电话: *
传真:
传真:
单位: *  
地址:
邮箱: *    
 
关闭
www.scaebuy.com
友情链接:幸运蛋蛋开奖  幸运蛋蛋导航网  幸运蛋蛋网址多少  幸运蛋蛋开奖直播网  幸运蛋蛋登陆  幸运蛋蛋  幸运蛋蛋  幸运蛋蛋网址  幸运蛋蛋app  幸运蛋蛋官方网址